Ribosome display,a newly discovered technique, is used to perform in vitro protein evolution so as to create proteins that can bind to a desired ligand. It overcomes the shortages of other traditional approaches of biological display.
What makes ribosome display different from other biological displays?
The process of ribosome display results in translated proteins that are associated with their mRNA progenitor which is used, as a complex, to bind to an immobilized ligand in a selection step. The mRNA-protein hybrids that bind well are then reverse transcribed to cDNA and their sequence amplified via PCR. The end result is a nucleotide sequence that can be used to create tightly binding proteins.
By having the protein progenitor attached to the complex, the processes of ribosome display skips the microarray/peptide bead/multiple-well sequence separation that is common in assays involving nucleotide hybridization and provides a ready way to amplify the proteins that do bind without decrypting the sequence until necessary. At the same time, this method relies on generating large, concentrated pools of sequence diversity without gaps and keeping these sequences from degrading, hybridizing, and reacting with each other in ways that would create sequence-space gaps.
Competing methods for protein evolution in vitro are phage display, yeast display, bacterial display, and mRNA display. As it is performed entirely in vitro, there are two main advantages in ribosome display over other selection technologies. First, the diversity of the library is not limited by the transformation efficiency of bacterial cells, but only by the number of ribosome and different mRNA molecules present in the test tube. Second, random mutations can be introduced easily after each selection round, as no library must be transformed after any diversification step. This allows facile directed evolution of binding proteins over several generations.
How Creative Biolabs makes itself different?
Thanks to its advantages, increasing groups are focusing on the research and development of ribosome display. Creative BioLabs has established to a unique ribosome display platform that is based on proprietary prokaryotic and eukaryotic ribosome display technologies. The ribosome display platform is a superior in vitro cell-free platform that generates peptide, protein and antibody libraries with an unparalleled diversity up to 1015; as a result, extremely high-affinity binders against a variety of targets can be isolated from the libraries. In particular, our ribosome display antibody libraries can produce antibodies with pM affinity, the highest affinity ever achieved by an antibody production technology.
To better service customers, Creative Biolabs has constructed various ribosome display libraries. They are able to generate libraries with a diversity of n*1013-15. Following libraries are frequently made:
    • Immune animal libraries
    • Combinatorial libraries
    • Random peptide libraries
    • Constrained flexible libraries
What's more, staff scientists of Creative Biolabs have extensive experience in screening both prokaryotic and eukaryotic ribosome display libraries. They have developed a robust process for optimization of a full range of ribosome display library screening technologies.
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What makes ribosome display different from other biological displays?
The process of ribosome display results in translated proteins that are associated with their mRNA progenitor which is used, as a complex, to bind to an immobilized ligand in a selection step. The mRNA-protein hybrids that bind well are then reverse transcribed to cDNA and their sequence amplified via PCR. The end result is a nucleotide sequence that can be used to create tightly binding proteins.
By having the protein progenitor attached to the complex, the processes of ribosome display skips the microarray/peptide bead/multiple-well sequence separation that is common in assays involving nucleotide hybridization and provides a ready way to amplify the proteins that do bind without decrypting the sequence until necessary. At the same time, this method relies on generating large, concentrated pools of sequence diversity without gaps and keeping these sequences from degrading, hybridizing, and reacting with each other in ways that would create sequence-space gaps.
Competing methods for protein evolution in vitro are phage display, yeast display, bacterial display, and mRNA display. As it is performed entirely in vitro, there are two main advantages in ribosome display over other selection technologies. First, the diversity of the library is not limited by the transformation efficiency of bacterial cells, but only by the number of ribosome and different mRNA molecules present in the test tube. Second, random mutations can be introduced easily after each selection round, as no library must be transformed after any diversification step. This allows facile directed evolution of binding proteins over several generations.
How Creative Biolabs makes itself different?
Thanks to its advantages, increasing groups are focusing on the research and development of ribosome display. Creative BioLabs has established to a unique ribosome display platform that is based on proprietary prokaryotic and eukaryotic ribosome display technologies. The ribosome display platform is a superior in vitro cell-free platform that generates peptide, protein and antibody libraries with an unparalleled diversity up to 1015; as a result, extremely high-affinity binders against a variety of targets can be isolated from the libraries. In particular, our ribosome display antibody libraries can produce antibodies with pM affinity, the highest affinity ever achieved by an antibody production technology.
To better service customers, Creative Biolabs has constructed various ribosome display libraries. They are able to generate libraries with a diversity of n*1013-15. Following libraries are frequently made:
    • Immune animal libraries
    • Combinatorial libraries
    • Random peptide libraries
    • Constrained flexible libraries
What's more, staff scientists of Creative Biolabs have extensive experience in screening both prokaryotic and eukaryotic ribosome display libraries. They have developed a robust process for optimization of a full range of ribosome display library screening technologies.
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